Slick Sheet: Project
Oak Ridge National Laboratory, with project co-funder National Energy Technology Laboratory, will conduct a Geographical Information System (GIS) spatial analysis to identify locations that (1) are within geological saline basins with potential for CO2 injection, (2) are close to existing NG pipelines or NG users for potential blending of RNG with NG, and (3) meet site-specific suitability criteria (e.g. slope, population density, and exclusion of protected lands, landslide and flood hazard, and EPA non-attainment areas).

Slick Sheet: Project
The Harvard University team will draw from efficient infrastructures for cheap sugar supply, maturing gas fermentation technology, and sophisticated strategies to engineer fatty acid metabolism. Current bioproduction platforms are limited regarding to carbon efficiency, product versatility or productivity. These platforms have left legacies that will aid Harvard in developing the next generation of carbon-efficient bioproduction, however.

Slick Sheet: Project
Environmental drivers that cause the production and flux of nitrous oxide (N2O) and spatial and temporal variability of soil carbon stocks create challenges to cost-effectively quantify N2O emissions and soil carbon stock changes at scale. Dagan aims to build, validate, and demonstrate an integrated system to reliably and economically measure field-level soil carbon and N2O emissions.

Slick Sheet: Project
The aviation industry requires energy-dense, carbon-based fuels, which are difficult to achieve biologically because of low yields and poor carbon conversion efficiency. The University of California, Berkeley, will engineer an approach to reach near 100% carbon conversion efficiency. A single organism can be limited by CO2 loss from core metabolic reactions.

Slick Sheet: Project
LanzaTech will create transformative technology to directly convert CO2 to ethanol at 100% carbon efficiency with technical assistance from the University of Michigan and Oak Ridge National Laboratory. The team will develop a novel biocatalyst that leverages affordable, renewable hydrogen (H2) to capture and fix CO2 directly into ethanol, a biofuel and feedstock for valuable products. The core inputs are carbon-free renewable energy, water, and CO2.

Slick Sheet: Project
The National Renewable Energy Laboratory, the University of Oregon, Genomatica, and DeNora will generate low-cost and low-carbon-intensity fatty acid methyl esters (FAME) feedstock to generate renewable diesel and sustainable jet fuel. The team’s biorefining concept uses electrochemically generated formate as a universal energy carrier to facilitate a carbon-optimized sugar assimilation fermentation to synthesize FAME without release of CO2.

Slick Sheet: Project
The Massachusetts Institute of Technology (MIT) has demonstrated a two-stage system where acetate is produced from CO2 and H2 via acetogenic fermentation in the first stage and then fed to the yeast reactor for converting acetate to lipids or alkanes. MIT proposes to reduce or eliminate CO2 generation during lipid production by (1) engineering an oleaginous yeast with the enzymes necessary to generate reducing equivalents from hydrogen, formic acid, or methanol, and (2) installing a carbon-conserving non-oxidative glycolysis.

Slick Sheet: Project
Invizyne Technologies proposes an electrically powered cell-free enzymatic approach for upgrading ethanol into more useful chemicals. Because carbon for 99% of organic chemicals is petroleum-derived, replacing petroleum carbon with carbon captured from the atmosphere could greatly mitigate carbon emissions. Atmospheric CO2 represents a potentially limitless source of inexpensive carbon, but there are significant challenges to converting captured CO2 into useful chemicals and fuels.

Slick Sheet: Project
ZymoChem will develop carbon- and energy-efficient biocatalysts capable of co-conversion of one-carbon molecules and biomass-derived substrates to a high-volume platform fuel and chemical intermediate. The team will demonstrate a carbon-conserving process decoupling growth and production. Most bioprocesses using microbes and renewable feedstocks to make fuels and chemicals are unprofitable, precluding their adoption on the industrial scale.

Slick Sheet: Project
Stanford University is developing a commercially attractive, scalable, carbon-negative technology for producing commodity biochemicals. Glucose, carbon dioxide (CO2), and electricity will provide the required atoms and energy for carbon-negative, energy-positive production. Instead of releasing CO2 into the atmosphere, this new approach will enable use of atmospheric CO2 and glucose obtained from cornstarch to produce renewable fuels and chemicals.